GBL Antibody

• Catalog Number: orb248173
• Category: Antibodies
• Description: GBL (G protein beta subunit-like), also called MLST8 (MTOR associated protein, LST8 homolog), is a subunit of the mTOR signaling complexes mTORC1 and mTORC2, which help regulate cell growth and survival in response to growth factor (mTORC1 & 2) and nutrient (mTORC1) signaling. It is required for the phosphorylation of S6 kinase and the signal adaptor protein Insulin receptor substrate 1 and S473 phosphorylation of AKT. GBL is also a regulator of TNFa-induced NF-kB activity, with overexpression inhibiting and down-regulation enhancing NF-kB signaling (Kim et al, 2008).
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: A recombinant protein fragment from the internal region of human GBL was used as the immunogen for this antibody.
• Dilution range: Western blot: 1-3ug/ml
• Purity: Protein A affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.5 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This GBL antibody is available for research use only.
• Entrez: 64223
• Storage: Aliquot the GBL antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Buffer/Preservatives: 1X PBS, pH 7.4
• Note: For research use only
• Application notes: Provided assay concentrations are suggestions only, GBL antibody titration may be required for optimal results.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of human samples using GBL antibody (primary antibody dilution at: at 1 ug/ml)