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Item 1 of 3
GBA Recombinant Rabbit Monoclonal Antibody
Catalog Number: orb1499373
| Catalog Number | orb1499373 |
|---|---|
| Category | Antibodies |
| Description | GBA Recombinant Rabbit Monoclonal Antibody |
| Target | GBA |
| Clonality | Recombinant |
| Species/Host | Rabbit |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Mouse, Rat |
| Form/Appearance | Liquid |
| Concentration | 1mg/ml |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Immunogen | KLH conjugated synthetic peptide derived from human GBA (450-536/536aa) |
| UniProt ID | P04062 |
| MW | 56 kDa |
| Tested applications | IF, IHC-Fr, IHC-P, WB |
| Dilution range | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 |
| Antibody Type | Recombinant Antibody |
| Clone Number | 4C2 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | Glucosidase beta; Acid beta glucosidase; Acid beta Read more... |
| Note | For research use only |
Blocking buffer: 5% NFDM/TBST, Primary Ab Dilution: 1:20000, Primary Ab incubation condition: 2 hours at room temperature, Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP), Lysate: 1: MCF-7, 2: Saos-2, 3: SH-SY5Y, 4: 4T1, 5: C6, Protein loading amount: 20 µg, Exposure time: 60 s, Predicted MW: 60 kDa, Observed MW: 60 kDa.
Tissue: Human kidney, Section type: Formalin-fixed & paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary Ab Dilution: 1:50, Primary Ab incubation condition: 1 hour at room temperature, Secondary Ab: SP Kit (Rabbit), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1499373.
Tissue: Human thyroid carcinoma, Section type: Formalin-fixed & paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary Ab Dilution: 1:50, Primary Ab incubation condition: 1 hour at room temperature, Secondary Ab: SP Kit (Rabbit), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1499373.
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