GBA Recombinant Rabbit Monoclonal Antibody

SKU: orb1499373

Description

GBA Recombinant Rabbit Monoclonal Antibody

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Isotype: IgG
• Clone No.: 4C2
• Immunogen: A synthesized peptide derived from human Lysosomal acid GCase (480-536/536aa)
• Target: GBA
• Molecular Weight: 56 kDa
• Purification: Affinity purified by Protein A

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

Glucosidase beta; Acid beta glucosidase; Acid beta-glucosidase; Alglucerase; beta glucocerebrosidase; BETA GLUCOSIDASE, ACID; beta-glucocerebrosidase; betaGC; D glucosyl N acylsphingosine glucohydrolase; D-glucosyl-N-acylsphingosine glucohydrolase; EC 3.2.1.45; Gba protein; GBA1; GC antibody GCase; GCB; GLCM_HUMAN; GLUC; Glucocerebrosidase(alt.); Glucocerebrosidase; GLUCOCEREBROSIDASE PSEUDOGENE; Glucosidase beta; Glucosidase, beta, acid; Glucosidase, beta; acid(includes glucosylceramidase); Glucosylceramidase; Imiglucerase; Lysosomal glucocerebrosidase.

Blocking buffer: 5% NFDM/TBST, Primary Ab Dilution: 1:20000, Primary Ab incubation condition: 2 hours at room temperature, Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP), Lysate: 1: MCF-7, 2: Saos-2, 3: SH-SY5Y, 4: 4T1, 5: C6, Protein loading amount: 20 µg, Exposure time: 60 s, Predicted MW: 60 kDa, Observed MW: 60 kDa.

Tissue: Human kidney, Section type: Formalin-fixed & paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary Ab Dilution: 1:50, Primary Ab incubation condition: 1 hour at room temperature, Secondary Ab: SP Kit (Rabbit), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1499373.

Tissue: Human thyroid carcinoma, Section type: Formalin-fixed & paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary Ab Dilution: 1:50, Primary Ab incubation condition: 1 hour at room temperature, Secondary Ab: SP Kit (Rabbit), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1499373.

Quick Database Links

Gene Symbol

GBA

UniProt

P04062