GATSL2 Antibody (N-term)

SKU: orb1932579

Description

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: WB - 1:1000, IHC-P-Leica - 1:250
• Reactivity: Human
• Predicted Reactivity: Mouse, Other, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB30525
• Immunogen: This GATSL2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 25-51 amino acids from the N-terminal region of human GATSL2. Antigen Region: 25-51 aa.
• Target: CASTOR2 {ECO:0000303|PubMed:26972053, ECO:0000312|HGNC:HGNC:37073}
• Molecular Weight: 36056 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

GATS-like protein 2, GATL2

Immunohistochemical analysis on paraffin-embedded human colon tissue was performed on the Leica BOND RXm. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:250) for 15 min at room temperature. Leica Bond Polymer Refine Detection was used as the secondary antibody.

Immunohistochemical analysis on paraffin-embedded human brain tissue was performed on the Leica BOND RXm. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:250) for 15 min at room temperature. Leica Bond Polymer Refine Detection was used as the secondary antibody.

All lanes: Anti-GATSL2 Antibody (N-term) at 1:1000 dilution. Lane 1: A549 whole cell lysate. Lane 2: Caco2 whole cell lysate. Lane 3: Hela whole cell lysate. Lane 4: MDA-MB-231 whole cell lysate. Lane 5: HepG2 whole cell lysate. Lane 6: U-2OS whole cell lysate. Lane 7: SK-BR-3 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 36 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

Gene Symbol

CASTOR2 {ECO:0000303|PubMed:26972053, ECO:0000312|HGNC:HGNC:37073}

UniProt

A6NHX0