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GATA3 Recombinant Rabbit Monoclonal Antibody
Description
Research Area
Images & Validation
−| Tested Applications | IHC-Fr, IHC-P, WB |
|---|---|
| Dilution Range | IHC-P=1:50-200, IHC-F=1:50-200, WB=1:500-2000 |
| Reactivity | Human, Mouse |
| Predicted Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Recombinant |
| Isotype | IgG |
| Clone No. | A1F12 |
| Immunogen | A synthesized peptide derived from human GATA3 (1-220/443aa) |
| Target | GATA3 |
| Molecular Weight | 49 kDa |
| Purification | Affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | PBS, Glycerol, rAlbumin. |
| Concentration | 1mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (GATA3) Monoclonal Antibody, Unconjugated (orb1974680) at 1:50 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
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GATA3 Recombinant Rabbit Monoclonal Antibody (orb1974680)
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