Recombinant GATA Binding Protein 3 Antibody / GATA-3

SKU: orb1716254

Description

GATA-3 (GATA binding protein 3) is a member of the GATA family of transcription factors. This 50kDa nuclear protein regulates the development and subsequent maintenance of a variety of human tissues, including hematopoietic cells, skin, kidney, mammary gland, and the central nervous system. Among several other roles, GATA-3 involved in luminal cell differentiation in the mammary gland and appears to control a set of genes involved in the differentiation and proliferation of breast cancer. The expression of GATA-3 is associated with the expression of estrogen receptor-alpha (ER) in breast cancer. GATA-3 has been shown to be a novel marker for bladder cancer. The study demonstrated that GATA-3 stained 67% of urothelial Carcinomas, but none of prostate or renal carcinomas.

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
• Reactivity: Human
• Application Notes:
  Optimal dilution of the recombinant GATA Binding Protein 3 antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Recombinant
• Isotype: Mouse IgG1, kappa
• Clone No.: rGATA3/3870
• Immunogen: Recombinant full-length human protein was used as the immunogen for the recombinant GATA Binding Protein 3 antibody.
• Purification: Protein A/G affinity
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin, 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

IHC staining of FFPE human urothelial carcinoma tissue with recombinant GATA Binding Protein 3 antibody (clone rGATA3/3870). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human lobular breast carcinoma tissue with recombinant GATA Binding Protein 3 antibody (clone rGATA3/3870). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Western blot testing of human MCF-7 cell lysate using recombinant GATA Binding Protein 3 antibody (clone rGATA3/3870). Observed molecular weight: ~50 kDa (full length), ~37 kDa (splice form).

Quick Database Links

UniProt

P23771