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GAPDH Monoclonal Antibody
Description
Images & Validation
−| Tested Applications | ELISA, FC, IF, IHC, IP, WB |
|---|---|
| Dilution range | WB:1:5000-1:1600000, IHC-P:1:50-1:500, IF:1:50-1:200, IP:1µl-2µl, FC:1:100-1:300 |
| Reactivity | Human, Rabbit, Rat |
Key Properties
−| Host | Mouse |
|---|---|
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone No. | 14C2F11 |
| Immunogen | Recombinant Human GAPDH protein (3-335AA) |
| Target | GAPDH |
| Purification | >95%, Protein G purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Preservative: 0.03% Proclin 300. Constituents: 50% Glycerol |
| Disclaimer | For research use only |
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Western blot analysis of A549(Lane 1), HT-29(Lane 2), Jurkat(Lane 3), U251(Lane 4), COLO205(Lane 5), MCF7(Lane 6), K562(Lane 7), Hela(Lane 8), 293T(Lane 9) whole cell lysate using GAPDH antibody
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GAPDH Monoclonal Antibody (orb243937)
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