GAPDH Antibody

SKU: orb1925326

Description

Purified Mouse Monoclonal Antibody (Mab)

Research Area

Metabolism Research

Images & Validation

• Tested Applications: WB
• Dilution Range: WB - 1:8000
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone No.: B3875EV614X41X88
• Immunogen: This GAPDH antibody is generated from a mouse immunized with a recombinant protein between 43-335 amino acids from human GAPDH.
• Target: GAPDH {ECO:0000303|PubMed:2987855, ECO:0000312|HGNC:HGNC:4141}
• Molecular Weight: 36053 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Glyceraldehyde-3-phosphate dehydrogenase, GAPDH, 1.2.1.12, Peptidyl-cysteine S-nitrosylase GAPDH, 2.6.99.-, GAPDH, GAPD

All lanes: Anti-GAPDH Antibody at 1:8000 dilution. Lane 1: C6 whole cell lysate. Lane 2: mouse brain lysate. Lane 3: NIH/3T3 whole cell lysate. Lane 4: Hela whole cell lysate. Lane 5: Jurkat whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 36 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

Gene Symbol

GAPDH {ECO:0000303|PubMed:2987855, ECO:0000312|HGNC:HGNC:4141}

UniProt

P04406