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Catalog Number | orb1331971 |
---|---|
Category | Antibodies |
Description | Mouse monoclonal antibody to GAPDH |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | OTI2D9 |
Tested applications | WB |
Reactivity | Canine, Human, Monkey, Mouse, Rat |
Isotype | IgG1 |
Immunogen | Full length human recombinant protein of human GAPDH (NP_002037) produced in HEK293T cell. |
Antibody Type | Primary Antibody |
Dilution range | WB 1:2000 |
Conjugation | Unconjugated |
MW | 35.9 kDa |
Target | GAPDH |
UniProt ID | P04406 |
NCBI | NM_002046 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Note | For research use only |
Application notes | For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. |
Expiration Date | 12 months from date of receipt. |
Figure from citation: Western blot analysis of GAPDH protein level by using anti-GAPDH antibody in fibroblasts, GAPDH used as loading control.
Figure from citation: Western blot analysis of GAPDH protein level by using anti-GAPDH antibody in various cell lines, GAPDH used as loading control.
Figure from citation: Western blot analysis of GAPDH protein level by using anti-GAPDH antibody in SAOS-2 cells. Dilution: 1:2000
Figure from citation: Western blot analysis of GAPDH protein level by using anti-GAPDH antibody in SAOS-2 cells. Dilution: 1:2000
Figure from citation: Western blot analysis of GAPDH protein level by using anti-GAPDH antibody in various cell lines, GAPDH used as loading control.
Figure from citation: Western blot analysis of GAPDH protein level by using anti-GAPDH antibody in SAOS-2 cells. Dilution: 1:2000
Figure from citation: Western Blot of GAPDH protein level by using anti-GAPDH antibody in HEK293 cells. Dilution: 1:500
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-GAPDH monoclonal antibody.
WB | |
Canine, Gallus, Monkey, Porcine, Rabbit, Sheep | |
Hamster, Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
ELISA, IF, IHC-Fr, IHC-P, WB | |
Mouse, Rat | |
Hamster, Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ICC, IF, IHC-Fr, IHC-P, WB | |
Gallus, Monkey, Zebrafish | |
Human, Mouse, Rat | |
Rabbit | |
Recombinant | |
Unconjugated |
ICC, IHC-P, IP, WB | |
Bacteria, Bovine, Canine, Drosophila, E. coli, Fish, Gallus, Hamster, Human, Insect, Mammal, Monkey, Mouse, Other, Plant, Porcine, Rabbit, Rat, Yeast, Zebrafish | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IF, IHC, WB | |
Canine, Human, Mouse, Porcine | |
Goat | |
Polyclonal | |
Unconjugated |