GAPDH Antibody

SKU: orb1247972

Description

GAPDH Antibody

Research Area

Metabolism Research

Images & Validation

• Tested Applications: ELISA, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Canine, Porcine
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 37kDa band observed in lysates of cell lines Hek293 and HeLa and in Human Liver, Tonsil, Mouse Spleen, and Rat Brain and Spleen lysates (calculated MW of 36.1kDa according to Human NP_002037.2 and 35.8kDa according to Mouse NP_032110.1 and Rat NP_058704.1). Recommended concentration: 0.01-0.5µg/mL. Primary incubation 1 hour at room temperature.Immunohistochemistry:Paraffin embedded Human Kidney. Recommended concentration: 2.5µg/mL.Immunofluorescence: Expression of the protein seen in the cytoplasm and vesicles of HeLa cells. Recommended concentration: 10µg/mL.

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Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-HQVVSSDFNSDT
• Target: GAPDH
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

GAPDH, glyceraldehyde-3-phosphate dehydrogenase, HGNC:4141, G3PD, GAPD, MGC88685, OTTHUMP00000174431, OTTHUMP00000174432, aging-associated gene 9 protein, glyceraldehyde 3-phosphate dehydrogenase

orb1247972 (0.01 ug/ml) staining of cell lines HEK293 (A) and HeLa (B) and Human Liver (C) and Tonsil (D) lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1247972 (0.01 ug/ml) staining of Mouse Spleen (A), (0.1 ug/ml) Rat Brain (B) and (0.5 ug/ml) Rat Spleen (C) (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1247972 (2.5 ug/ml) staining of paraffin embedded Human Kidney.

orb1247972 Negative Control showing staining of paraffin embedded Human Kidney, with no primary antibody.

orb1247972 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing cytoplasmic and vesicle staining.

Quick Database Links

Gene Symbol

GAPDH

UniProt

P04406

RefSeq (Protein):NP_002037.2