GAPDH Antibody

• Catalog Number: orb1247971
• Category: Antibodies
• Description: GAPDH Antibody
• Target: GAPDH
• Clonality: Polyclonal
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Canine
• Form/Appearance: Liquid
• Concentration: 500 ug/mL
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Immunogen: The immunogen for this antibody is: C-GVNHEKYDNSLK
• UniProt ID: P04406
• Tested applications: ELISA, IF, IHC, WB
• Application notes: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 37kDa band observed in Human Liver, Testis and Tonsil, and in Mouse Liver and Rat Heart lysates, and in cell lysates of HeLa and NIH3T3 (calculated MW of 36.1kDa according to Human NP_002037.2, Mouse NP_032110.1, and Rat NP_058704.1). Recommended concentration: 0.03-0.1ug/ml. Primary incubation 1 hour at room temperature. This product has been successfully used in WB on Human: PMID: 29953622 and PMID: 29158223.Immunohistochemistry:In paraffin embedded Human Liver shows textured cytoplasm staining in hepatocytes. Recommended concentration: 2ug/ml.Immunofluorescence: Strong expression of the protein seen in the cytoplasm of U251 and HeLa cells. Recommended concentration: 5-10ug/ml.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: GAPDH, glyceraldehyde-3-phosphate dehydrogenase, H
• Note: For research use only
• NCBI: NP_002037.2, NP_001243728.1

orb1247971 (0.1 ug/ml) staining of Human Liver (A), (0.03 ug/ml) Testes (B) and Tonsil (C) lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1247971 (2 ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

orb1247971 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing cytoplasmic and plasma membrane staining.

orb1247971 (0.1 ug/ml) staining of Mouse Liver (A) and (0.03 ug/ml) Rat Heart (B) lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1247971 (0.03 ug/ml) staining of HeLa (A) and NIH3T3 (B) cell lysate (35 ug protein in RIPA buffer). Detected by chemiluminesce.

orb1247971 Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing cytoplasmic and vesicle staining.