GAPDH Antibody

• Catalog Number: orb1240311
• Category: Antibodies
• Description: GAPDH Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: GAPDH antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human GAPDH.
• Concentration: 1 mg/mL
• Dilution range: GAPDH antibody can be used for the detection of GAPDH by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human and mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: GAPDH
• NCBI: P04406
• Storage: GAPDH antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: GAPDH Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: GAPDH Antibody: Glyceraldehyde-3-phosphate dehydro
• Note: For research use only
• Application notes: GAPDH antibody can be used for the detection of GAPDH by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human and mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of GAPDH in A431, Daudi, HepG2, HL60, Jurkat, Human kidney, Mouse lung, and Chicken liver lysate with GAPDH antibody 0.5 µg/mL.

Immunohistochemical staining of GAPDH in HeLa cells at a dilution of 10 µg/mL.

Immunofluorescence of GAPDH in HeLa cells with GAPDH antibody at 20 µg/mL.