GAPDH Antibody

• Catalog Number: orb1239765
• Category: Antibodies
• Description: GAPDH Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-P, WB
• Predicted Reactivity: Bovine, Gallus, Porcine, Rabbit, Sheep
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: GAPDH antibody was raised against a 16 amino acid synthetic peptide from near the amino-terminus of human GAPDH.The immunogen is located within amino acids 60 - 110 of GAPDH.
• Concentration: 1 mg/mL
• Dilution range: GAPDH antibody can be used for the detection of GAPDH by Western blot at 0. 125 - 0.5 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 10 μg/mL.Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: GAPDH
• UniProt ID: P04406
• NCBI: P04406
• Storage: GAPDH antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: GAPDH Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: GAPDH Antibody: G3PD, GAPD, HEL-S-162eP, CDABP0047
• Note: For research use only
• Application notes: GAPDH antibody can be used for the detection of GAPDH by Western blot at 0. 125 - 0.5 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 10 μg/mL.Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of GAPDH in HeLa cell lysate with GAPDH antibody at (A) 0.125, (B) 0.25 and (C) 0.5 µg/mL.

Immunohistochemistry of GAPDH in human liver tissue with GAPDH antibody at 10 µg/mL.

Immunofluorescence of GAPDH in HeLa cells with GAPDH antibody at 10 µg/mL.