Plakoglobin Antibody / Gamma Catenin

SKU: orb385949

Description

Plakoglobin, or gamma Catenin, is a common junctional plaque protein. The membrane-associated plaques are architectural elements in an important strategic position to influence the arrangement and function of both the cytoskeleton and the cells within the tissue. The presence of Plakoglobin in both the desmosomes and in the intermediate junctions suggests that it plays a central role in the structure and function of submembranous plaques. Acts as a substrate for VE-PTP and is required by it to stimulate VE-cadherin function in endothelial cells. Can replace beta-catenin in E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton (By similarity). [UniProt]

Research Area

Cardiovascular Disease, Epigenetics

Images & Validation

• Tested Applications: FACS, IF, IHC-P, WB
• Dilution Range: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,Immunofluorescence: 1-2 ug/ml,Flow cytometry: 1-2ug/million cells
• Reactivity: Human, Mouse, Rat
• Application Notes:
  The stated application concentrations are suggested starting amounts. Titration of the Plakoglobin antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1, kappa
• Clone No.: 15F11
• Immunogen: Recombinant full length chicken gamma Catenin was used as the immunogen for the Plakoglobin antibody.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Immunofluorescent staining of human HeLa cells with Plakoglobin antibody (clone 15F11, green) and Reddot nuclear stain (red).

Western blot testing of human 1) HT20, 2) HEK293, 3) A431, 4) MCF7, 5) HEPG2 and 6) A549 cell lysate with Plakoglobin antibody (clone 15F11). Predicted molecular weight: 80-87 kDa.

Flow cytometry testing of PFA-fixed human MCF7 cells with Plakoglobin antibody (clone 15F11); Red = isotype control, Blue = Plakoglobin antibody.

Western blot testing of human 1) HeLa and 2) SK-BR-3 cell lysate with Plakoglobin antibody (clone 15F11). Predicted molecular weight: 80-87 kDa.

IHC staining of FFPE human cervix with Plakoglobin antibody (clone 15F11). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Quick Database Links

UniProt

P14923