Gamma Catenin Antibody / Plakoglobin

• Catalog Number: orb385949
• Category: Antibodies
• Description: Plakoglobin, or gamma Catenin, is a common junctional plaque protein. The membrane-associated plaques are architectural elements in an important strategic position to influence the arrangement and function of both the cytoskeleton and the cells within the tissue. The presence of Plakoglobin in both the desmosomes and in the intermediate junctions suggests that it plays a central role in the structure and function of submembranous plaques. Acts as a substrate for VE-PTP and is required by it to stimulate VE-cadherin function in endothelial cells. Can replace beta-catenin in E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton (By similarity). [UniProt]
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 15F11
• Tested applications: FACS, IF, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Mouse IgG1, kappa
• Immunogen: Recombinant full length chicken gamma Catenin was used as the immunogen for the Plakoglobin antibody.
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This Plakoglobin antibody is available for research use only.
• UniProt ID: P14923
• Storage: Store the Plakoglobin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: The stated application concentrations are suggested starting amounts. Titration of the Plakoglobin antibody may be required due to differences in protocols and secondary/substrate sensitivity.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of human HT20(lane 1), HEK293(lane 2), A431(lane 3), MCF7(lane 4), HEPG2(lane 5) and A549(lane 6) cell lysate using Plakoglobin antibody