Gamabufotalin

SKU: orb1222243

Description

Gamabufotalin , a major bufadienolide of Chansu, has been used for treatment of COX-2-mediated diseases and cancer therapy due to its desirable metabolic stability and less adverse effect.

Key Properties

• CAS Number: 465-11-2
• MW: 402.53
• Purity: >98% (HPLC)
• Formula: C₂₄H₃₄O₅
• SMILES: CC12CCC(CC1CCC3C2C(CC4(C3(CCC4C5=COC(=O)C=C5)O)C)O)O
• Target: 5-HT Receptor
• Solubility: In Vitro: DMSO : 50 mg/mL (124.22 mM)

Bioactivity

• In Vivo:
  Animal model: A549-xenografts mice. Dosage: 5 and 20 mg/kg/day. Administration: i.p. , for 17 days. Result: Reduced tumor volume and the tumor weights. Decreased COX-2 and p-p65 level in tumors.
• In Vitro:
  Apoptosis Analysis Cell line: A549 cells. Concentration: 0-100 nM. Incubation time: 12-48 h. Result: Increase the expression levels of the cleaved caspase-3, caspase-9 and PARP. Induced the release of cyt c from mitochondria to cytosol. Immunofluorescence Cell line: A549 cells. Concentration: 0-100 nM. Incubation time: 12-48 h. Result: Inhibited translocation of the NF-κB p65/p50 proteins from cell cytoplasm to nucleus, and induced p300 into the cytoplasm.

Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Quick Database Links

Gene Symbol

5-HT Receptor