Galectin 8 Recombinant Rabbit Monoclonal Antibody

• Catalog Number: orb612219
• Category: Antibodies
• Description: Galectin 8 Recombinant Rabbit Monoclonal Antibody
• Target: LGALS8
• Clonality: Recombinant
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated synthetic peptide derived from human Galectin 8
• UniProt ID: O00214
• MW: 35 kDa
• Tested applications: ICC, IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:50-200, IF=1:100-500
• Antibody Type: Primary Antibody
• Clone Number: 1A4
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Gal 8; Gal-8; Gal8; Galectin-8; LEG8_HUMAN; LGAL S
• Research Area: Cancer Research, Cell Adhesion, Cell Biology
• Note: For research use only

Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma), Antigen retrieval by microwaving in EDTA buffer (Ph8.0) for 5 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Galectin 8) Monoclonal Antibody, Unconjugated (orb612219) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.