LGALS3 Antibody

SKU: orb1272725

Description

LGALS3 Antibody

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Reactivity: Human
• Application Notes:
  Sandwich ELISA:To detect Galectin-3 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Human Galectin-3 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Galectin-3. Western Blot:To detect Galectin-3 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant Galectin-3 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Immunohistochemistry:This antibody stained formalin-fixed paraffin-embedded sections of human pancreas infiltrating ductal adenocarcinoma tissue. The recommended concentration is 31.25 ng/mL with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Optimal results for these conditions were achieved with heat induced antigen retrieval with a pH 6.0 sodium citrate buffer. Optimal concentrations and conditions may vary.

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Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant Galectin-3. Human Galectin-3 specific antibody was purified by affinity chromatography employing immobilized Galectin-3 matrix.
• Target: LGALS3
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

L31, GAL3, MAC2, CBP35, GALBP, GALIG, LGALS2, Galectin-3, 35 kDa lectin, Gal-3

Quick Database Links

Gene Symbol

LGALS3

UniProt

P17931

RefSeq:P17931