Galectin 1 Antibody [GAL1/1831]

• Catalog Number: orb1252358
• Category: Antibodies
• Description: Galectin 1 Antibody [GAL1/1831]
• Target: Lgals1
• Clonality: Monoclonal
• Isotype: IgG1, lambda
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid
• Concentration: 0.2 mg/mL
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Purification: Protein G affinity chromatography
• Immunogen: A human partial recombinant protein corresponding to amino acids 12-108 was used as the immunogen for the Galectin 1 antibody.
• UniProt ID: P09382
• Tested applications: ELISA, FC, IF, IHC-P, WB
• Application notes: ELISA: 2-4 ug/ml; order the BSA-free formulation for coatingIF: 1-2 ug/mlFlow Cytometry: 0.5-1 ug/million cells in 0.1mlWB: 0.5-1 ug/mlIHC (FFPE): 0.5-1 ug/ml for 30 min at RTTitering of the Galectin 1 antibody may be required for optimal performance.
• Antibody Type: Primary Antibody
• Clone Number: GAL1/1831
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Galectin-1, Gal-1, 14 kDa laminin-binding protein,
• Research Area: Cancer Biology, Cell Biology, Neuroscience, Signal
• Note: For research use only

IHC testing of FFPE human prostate carcinoma with Galectin 1 antibody (clone GAL1/1831). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.

Western blot testing of human 1) HeLa, 2) K562 and 3) HEK293 cell lysate with Galectin 1 antibody (clone GAL1/1831). Expected molecular weight ~14 kDa.