GALE Antibody (Center)

SKU: orb37163

Description

Peptide affinity purified rabbit polyclonal antibody.

Images & Validation

• Tested Applications: FC, IF, IHC-P, WB
• Dilution range: WB: 1:1000, IHC-P: 1:50-100, FACS: 1:10-50, IF/ICC: 1:10-50
• Reactivity: Human
• Predicted Reactivity: Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB21189
• Immunogen: Synthetic Peptide
• Target: GALE
• Molecular Weight: 38282
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Disclaimer: For research use only

Alternative Names

Anti-UDP-glucose 4-epimerase antibody, anti-UDP-galactose 4-epimerase antibody, anti-Galactowaldenase antibody, anti-GALE antibody

Western blot analysis of GALE Antibody (Center) in A375 cell line lysates (35 ug/lane). GALE (arrow) was detected using the purified Pab.

Confocal immunofluorescent analysis of GALE Antibody (Center) with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

GALE Antibody (Center) flow cytometry analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Formalin-fixed and paraffin-embedded human skin tissue reacted with GALE Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Quick Database Links

Gene Symbol

GALE

UniProt

Q14376

RefSeq (Protein):NP_001008217.1, NP_000394.2, NP_001121093.1