Galangin

SKU: orb1303934

Description

Galangin

Research Area

Cell Biology, Metabolism Research, Signal Transduction

Key Properties

• CAS Number: 548-83-4
• MW: 270.24
• Purity: 99.93%
• Formula: C₁₅H₁₀O₅
• SMILES: Oc1cc(O)c2c(c1)oc(-c1ccccc1)c(O)c2=O
• Target: Autophagy,Cytochromes P450,NF-κB,ERK
• Solubility: Chloroform, Dichloromethane, Ethyl Acetate, Acetone:Soluble;10% DMSO+40% PEG300+5% Tween 80+45% Saline:5 mg/mL (18.5 mM);DMSO:237 mg/mL (877 mM)

Bioactivity

• In Vitro:
  Through thin-layer chromatography, it has been demonstrated that Galangin impede the catabolic decomposition of DMBA in a dose-dependent fashion and obstruct the generation of DMBA-DNA adducts, thereby averting DMBA-related suppression of cell proliferation. Additionally, Galangin robustly curtails CYP1A1 enzyme activity in a dose-responsive manner wit In both intact cells and DMBA-exposed cell-derived microsomes, as ascertained through ethoxyresorufin-O-deethylase activity assays the Inhibition of CYP1A1 by Galangin occurs through a noncompetitive mechanism, as evidenced by double-reciprocal plot analysis. Moreover, Galangin elevates CYP1A1 mRNA levels, suggesting potential aryl hydrocarbon receptor agonism, yet it counteract the DMBA or TCDD-triggered upregulation of CYP1A1 mRNA and promoter-driven transcriptio. furthermore , Galangin administration hampers cell proliferation while propelling autophagy at 130 μM and apoptosis at 370 μM. Specifically, in HepG2 cells, Galangin induces autophagosome accumulation, enhances microtubule-associated protein light chain 3 levels, and augment the proportion of vacuolated cells, alongside a surge in p53 expression the Galangin-induced autophagic response is mitigated by p53 Inhibition in HepG2 cells, while p53 overexpression in Hep3B cells normalize the elevated vacuole presenc.
• Cell Research:
  Cells (5. × 03) are seeded and treated with different concentration of galangin for different periods of time in 96-well plates the number of viable cells in Each well is determined by adding 10 μL of 5 mg mL MTT solution. Followin the 4 hour incubation at 37℃he cells are dissolved in a 100 μL solution containing 20% SDS and 50% dimethy formamide the optical densities are quantified at a test wavelength of 570 nM with a reference wavelength of 630 nM using a Varioskan Flash Reader spectrophotometer.

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Disclaimer: For research use only

Alternative Names

Autophagy, 3,5,7-Trihydroxyflavone, CYPs, Cytochrome P450, ERK, Galangin, Inhibitor, NF-κB, NFκB, NFkB, NF-kB, inhibit, Norizalpinin

Compound Identifiers

PubChem CID

5281616