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Description
Research Area
Neuroscience
Images & Validation
−Item 1 of 1
| Tested Applications | FC, IP, RIA, WB |
|---|---|
| Dilution Range | Optimal antibody dilution should be determined by titration, however as a guideline try WB: Use a concentration of 1 µg/ml. Predicted molecular weight: 65 kDa |
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Host | Mouse |
|---|---|
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone No. | 144 |
| Immunogen | Synthetic peptide: GSGFWSFGSEDGSGDSEN conjugated to KLH, corresponding to amino acids 4-22 of Human GAD65 |
| Target | GAD65 |
| Purification | Affinity purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Purified antibody containing PBS 0.1% sodium azide; |
| Concentration | 1mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−65 kDa glutamic acid decarboxylase antibody;DCE 2 antibody;DCE2 antibody;GAD 2 antibody;GAD 65antibody;GAD-2 antibody;GAD-65 antibody;GAD2 antibody;Glutamate Decarboxylase 2 (pancreatic islets and brain65kDa) antibody;Glutamate Decarboxylase 2 antibody;Glutamate Decarboxylase 65 antibody;Glutamate decarboxylase65 kDa isoform antibody;Glutamic Acid Decarboxylase 2 antibody;Glutamic Acid Decarboxylase 65antibody
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Western blot analysis of extracts of human brain tissue lysate using NGAD65 antibody
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Protocol Information
WB
Western Blot (IB, immunoblot)
FC
Flow Cytometry
IP
Immunoprecipitation
RIA
Radioimmunoassay
GAD65 Monoclonal Antibody (orb76630)
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