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Catalog Number | orb1246603 |
---|---|
Category | Antibodies |
Description | GABRA4 Antibody |
Clonality | Polyclonal |
Tested applications | ELISA, IHC, WB |
Predicted Reactivity | Bovine |
Reactivity | Human |
Immunogen | The immunogen for this antibody is: C-EKAKRKTSKPPQE |
Antibody Type | Primary Antibody |
Concentration | 500 ug/mL |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
Target | GABRA4 |
UniProt ID | P48169 |
NCBI | NP_000800.2 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | GABRA4, gamma-aminobutyric acid (GABA) A receptor, Read more... |
Note | For research use only |
Application notes | Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx. 70kDa band observed in Human Brain (Cerebellum and Frontal Cortex) lysates (calculated MW of 61.6kDa according to NP_000800.2). Recommended concentration: 0.1-0.3ug/ml.Immunohistochemistry:In paraffin embedded Human Cortex shows patchy staining near or at the neuronal cell membrane.. Recommended concentration, 2-4ug/ml. |
Expiration Date | 12 months from date of receipt. |
orb1246603 (0.1 ug/ml) staining of Human Frontal Cortex lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
orb1246603 (2.5 ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
IF, IH, WB | |
Human, Mouse, Primate, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IH, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |