GABARAP Antibody: Biotin

• Catalog Number: orb377279
• Category: Antibodies
• Description: Rabbit polyclonal antibody against GABARAP conjugated to Biotin.
• Target: GABARAP
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Biotin
• Reactivity: Human, Mouse
• Concentration: 1 mg/ml
• Buffer/Preservatives: 136.36mM Ethanolamine, and 9.55mM Sodium Bicarbonate in 95.45% PBS
• Purification: Peptide Affinity Purified
• Immunogen: Synthetic peptide from the N-terminal of human GABARAP
• UniProt ID: O95166
• MW: 14-16kDa
• Tested applications: ELISA, ICC, IF, IHC, WB
• Dilution range: WB (1:1000); ICC/IF (1:100)
• Application notes: A 1:1000 dilution of SPC-620 was sufficient for detection of GABARAP on mouse kidney lysates using Goat anti-rabbit IgG:HRP as the secondary antibody.
• Storage: Conjugated antibodies should be stored according to the product label
• Alternative names: ATG8A antibody, GABARAP A antibody, GABA(A) Recept
• Research Area: Cancer Biology, Cell Biology, Neuroscience
• Note: For research use only
• Entrez: 11337
• NCBI: NP_009209.1
• Expiration Date: 12 months from date of receipt.

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-GABARAP Polyclonal Antibody. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-GABARAP Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Endomembrane System, Cytoplasm, Golgi Apparatus Membrane, Cytoplasmic Vesicle. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) GABARAP Antibody (D) Composite.

Western blot analysis of Human Embryonic kidney epithelial cell line (HEK293T) lysate showing detection of ~13.9kDa GABARAP protein using Rabbit Anti-GABARAP Polyclonal Antibody. Lane 1: MW Ladder. Lane 2: Human 293T (20 μg). Load: 20 μg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-GABARAP Polyclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~13.9kDa. Other Band (s): ~40kDa (Complex).

Western blot analysis of Mouse Kidney showing detection of ~13.9kDa GABARAP protein using Rabbit Anti-GABARAP Polyclonal Antibody. Lane 1: MW Ladder. Lane 2: Mouse Kidney (20 μg). Load: 20 μg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-GABARAP Polyclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~13.9kDa. Other Band (s): ~40kDa (Complex).