GABA B Receptor 2 Antibody

SKU: orb67490

Description

Mouse Anti-Rat GABAB Receptor 2 Monoclonal IgG1

Research Area

Neuroscience

Images & Validation

• Tested Applications: ICC, IF, IHC, WB
• Dilution Range: WB (1:1000)
• Reactivity: Human, Mouse, Rat
• Application Notes:
  1 µg/ml of SMC-402 was sufficient for detection of GABA(B)R2 in 20 µg of rat brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: N81/2 (Formerly sold as S81-2)
• Immunogen: Fusion protein amino acids 861-912 of rat GABA(B)R2
• Target: GABA B Receptor 2
• Molecular Weight: 105kDa
• Purification: Protein G Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS pH 7.4, 50% glycerol, 0.09% sodium azide Storage buffer changes when conjugated
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

GABA B receptor 2, GABA-B receptor 2, GABA-B-R2, GABA-BR2, GABAB R2, GABABR2, GABBR 2, GABBR2, GABR2_HUMAN, Gamma aminobutyric acid (GABA) B receptor 2, Gamma aminobutyric acid B receptor 2, Gamma aminobutyric acid GABA B receptor 2, Gamma aminobutyric acid type B receptor subunit 2, Gb 2, Gb2, GPR 51, GPR51, G protein coupled receptor 51, G-protein coupled receptor 51, GPRC 3B, GPRC3B, HG 20, HG20, HRIHFB2099, Metabotropic GABA B receptor subtype 2, BcDNA:GH07312, CG6706, CT20836, D GABA[[B]]R2, D Gaba2, FLJ36928, GH07312, OTTHUMP00000021776, OTTHUMP00000063797, R2 SUBUNIT

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GABA-B Receptor 2 Monoclonal Antibody, Clone N81/2. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-GABA-B Receptor 2 Monoclonal Antibody at 1:100 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) GABA-B Receptor 2 Antibody (D) Composite.

Western Blot analysis of Rat Brain Membrane showing detection of ~105 kDa GABA B Receptor 2 protein using Mouse Anti-GABA B Receptor 2 Monoclonal Antibody, Clone N81/2. Lane 1: MW Ladder. Lane 2: Rat Brain Membrane (10 μg). Load: 10 μg. Block: 5% milk. Primary Antibody: Mouse Anti-GABA B Receptor 2 Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: TMB solution for 10 min at RT. Predicted/Observed Size: ~105 kDa.

Quick Database Links

Gene Symbol

GABA B Receptor 2

UniProt

O88871

NCBI Gene ID

83633

RefSeq (Protein):NP_113990.1