GABA B Receptor 1 Antibody (APC)

SKU: orb149877

Description

Mouse monoclonal to GABA-B R 1 (APC). GABA (gamma-aminobutyric acid) is the primary inhibitory neurotransmitter in the central nervous system and interacts with three different receptors: GABA(A), GABA(B) and GABA(C) receptor. The ionotropic GABA(A) and GABA(C) receptors are ligand-gated ion channels that produce fast inhibitory synaptic transmission. In contrast, the metabotropic GABA(B) receptor is coupled to G proteins that modulate slow inhibitory synaptic transmission. Functional GABA(B) receptors form heterodimers of GABA(B)R1 and GABA(B)R2 where GABA(B)R1 binds the ligand and GABA(B)R2 is the primary G protein contact site. Two isoforms of GABA(B)R1 have been cloned: GABA(B)R1a is a 130 kD protein and GABA(B)R1b is a 95 kD protein. G proteins subsequently inhibit adenyl cylase activity and modulate inositol phospholipid hydrolysis. GABA(B) receptors have both pre- and postsynaptic inhibitions: presynaptic GABA(B) receptors inhibit neurotransmitter release through suppression of high threshold calcium channels, while postsynaptic GABA(B) receptors inhibit through coupled activation of inwardly rectifying potassium channels. In addition to synaptic inhibition, GABA(B) receptors may also be involved in hippocampal long-term potentiation, slow wave sleep and muscle relaxation..

Research Area

Neuroscience

Images & Validation

• Tested Applications: ICC, IF, WB
• Dilution range: WB (1:1000)
• Reactivity: Human, Mouse, Rat
• Application Notes:
  1 µg/ml of SMC-403 was sufficient for detection of GABA(B)R1 in 20 µg of rat brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: N93A/49 (Formerly sold as S93A-49)
• Immunogen: Fusion protein amino acids 873-977 (cytoplasmic C-terminus) of rat GABA(B)R1
• Target: GABA B Receptor 1
• Molecular Weight: 115kDa
• Purification: Protein G Purified
• Conjugation: APC

Storage & Handling

• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 95.46mM Phosphate, 2.48mM MES and 2mM EDTA
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

GABA-B receptor 1, GABA-B-R1, GABR1_HUMAN, Gamma aminobutyric acid receptor 1, GB1, GPRC3A, Gamma-aminobutyric acid type B receptor subunit 1, GABBR1

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GABA-B Receptor 1 Monoclonal Antibody, Clone N93A/49. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-GABA-B Receptor 1 Monoclonal Antibody at 1:50 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) GABA-B Receptor 1 Antibody (D) Composite.

Western Blot analysis of Rat brain membrane lysate showing detection of GABA B Receptor 1 protein using Mouse Anti-GABA B Receptor 1 Monoclonal Antibody, Clone N93A/49. Primary Antibody: Mouse Anti-GABA B Receptor 1 Monoclonal Antibody at 1:1000.

Quick Database Links

Gene Symbol

GABA B Receptor 1

UniProt

Q9Z0U4

NCBI Gene ID

81657

RefSeq (Protein):NP_112290.2