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Item 1 of 3
GABA-A Receptor Delta Antibody: Biotin
Catalog Number: orb148892
| Catalog Number | orb148892 |
|---|---|
| Category | Antibodies |
| Description | Mouse monoclonal to GABA-A R Delta (Biotin). The GABA-A receptor is a member of the superfamily of fast acting ligand-gated ion channels. The individual subunits of these receptors have similar sequences and structural features. GABA-A receptors are the major fast inhibitory neurotransmitter gated ion channels in the brain.. |
| Species/Host | Mouse |
| Clonality | Monoclonal |
| Clone Number | N151/3 (Formerly sold as S151-3) |
| Tested applications | ELISA, ICC, IF, IHC, WB |
| Reactivity | Human, Mouse, Rat |
| Isotype | IgG2a |
| Immunogen | Synthetic peptide amino acids 15-34 (N-terminus) of rat GABA-A-R-Delta |
| Concentration | 1 mg/ml |
| Dilution range | WB (1:1000), IHC (1:1000), ICC/IF (1:100) |
| Conjugation | Biotin |
| MW | 55kDa |
| Target | GABA A Receptor |
| Entrez | 29689 |
| UniProt ID | P18506 |
| NCBI | NP_058985.1 |
| Storage | Conjugated antibodies should be stored according to the product label |
| Buffer/Preservatives | 136.36mM Ethanolamine, and 9.55mM Sodium Bicarbonate in 95.45% PBS |
| Alternative names | GABAA-RD antibody, Gabrd antibody, MGC105467 antib Read more... |
| Note | For research use only |
| Application notes | 2 µg/ml of SMC-345 was sufficient for detection of Delta1 GABA-A receptor in 10 µg of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody. |
| Expiration Date | 12 months from date of receipt. |
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GABA-A Receptor Delta Monoclonal Antibody, Clone N151/3. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-GABA-A Receptor Delta Monoclonal Antibody at 1:100 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) GABA-A Receptor Delta Antibody (D) Composite.
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GABA A Receptor Monoclonal Antibody, Clone N151/3. Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20°C. Primary Antibody: Mouse Anti-GABA A Receptor Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Diffuse cytoplasm and dull nuclei.
Western Blot analysis of Rat Cell line lysates showing detection of GABA A Receptor protein using Mouse Anti-GABA A Receptor Monoclonal Antibody, Clone N151/3. Load: 15 μg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-GABA A Receptor Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
