Gab1 Antibody (Ascites)

SKU: orb652191

Description

Mouse monoclonal antibody to GAB1

Research Area

Cell Biology

Images & Validation

• Tested Applications: WB
• Dilution Range: WB: 1:1000
• Reactivity: Human

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone No.: B3CV3191
• Immunogen: This Gab1 antibody is generated from mice immunized with a GST fusion protein encoding full length human GAB1.
• Target: GAB1
• Molecular Weight: 76616
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Anti-GRB2-associated-binding protein 1 antibody, anti-GRB2-associated binder 1 antibody, anti-Growth factor receptor bound protein 2-associated protein 1 antibody, anti-GAB1 antibody

Rescue of the JNK pathway by expression of wild-type Gab1 in Gab1-/- cells. Gab1-/- cells were transiently transfected with 1, 2, or 4 of the expression construct for human Gab1 cDNA, using the GenePORTER 2 transfection reagent. After incubation, the cells were irradiated with UV-B light at 400 J/m2 or left untreated. Exogenous expression of Gab1 in transfected cells was confirmed by anti-Gab1 immunoblot analysis with wild-type cells as positive control. +/+, wild-type cells; -/-, Gab1-/- cells.

Wild-type (+/+) and Gab1-/- (-/-) cells were heat shocked (HS) at 42°C for 1 h or irradiated with UV-B light (400 J/m2) and then incubated at 37°C for 1 h. Cell lysates were prepared and subjected to immunoprecipitation (IP) with an anti-JNK2 Antibody. The immunoprecipitates were resolved by SDS- PAGE and immunoblotted (IB) with anti-Gab1 Antibody.

Quick Database Links

Gene Symbol

GAB1

UniProt

Q13480

RefSeq (Protein):NP_002030.2, NP_997006.1