GAA Antibody (N-term)

• Catalog Number: orb1937382
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB18979
• Tested applications: IHC, IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Dilution range: WB: 1:2000, WB: 1:1000, WB: 1:1000, WB: 1:1000, IHC-P-Leica: 1:500, IHC-P-Leica: 1:500
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 105324 Da
• Target: This GAA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 174-203 amino acids from the N-terminal region of human GAA.
• UniProt ID: P10253
• NCBI: NP_001073271.1, NP_000143.2, NP_001073272.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Lysosomal alpha-glucosidase, Acid maltase, Aglucos
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

All lanes: Anti-GAA Antibody (N-term) at 1:1000 dilution. Lane 1: Hela whole cell lysate. Lane 2: MCF-7 whole cell lysate. Lane 3: SW620 whole cell lysate. Lane 4: A549 whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 105 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Anti-GAA Antibody (N-term) at 1:1000 dilution + Human placenta tissue lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 105 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Immunohistochemical analysis of paraffin-embedded Human liver tissue performed on the Leica BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary Antibody.

Immunohistochemical analysis of paraffin-embedded Human placenta tissue performed on the Leica BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary Antibody.

Western blot analysis of lysate from Jurkat cell line, using GAA Antibody (N-term). diluted at 1:2000. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary Antibody. Lysate at 20 ug.

Western blot analysis of lysates from A549, MCF-7, SW620, PC-3 cell line (from left to right), using GAA Antibody (N-term). diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary Antibody. Lysates at 20 ug per lane.