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Item 1 of 4
G6PC Antibody (Center)
Catalog Number: orb1930612
| Catalog Number | orb1930612 |
|---|---|
| Category | Antibodies |
| Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Clone Number | RB30027 |
| Tested applications | FC, IHC-P, WB |
| Reactivity | Human |
| Isotype | Rabbit IgG |
| Antibody Type | Primary Antibody |
| Dilution range | WB: 1:1000, WB: 1:1000, IHC-P: 1:50~100, FC: 1:10~50 |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Conjugation | Unconjugated |
| MW | 40484 Da |
| Target | This G6PC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 123-149 amino acids from the Central region of human G6PC. |
| UniProt ID | P35575 |
| NCBI | NP_000142.2, NP_001257326.1 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | Glucose-6-phosphatase, G-6-Pase, G6Pase, Glucose-6 Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
G6PC Antibody (Center) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
G6PC Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the G6PC Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Western blot analysis of G6PC Antibody (Center) in K562 cell line lysates (35 ug/lane).G6PC (arrow) was detected using the purified Pab.
Anti-G6PC Antibody (Center) at 1:1000 dilution + HepG2 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 40 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
