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FZD6 Antibody
SKU: orb1672347
Description
Images & Validation
−Item 1 of 4
| Tested Applications | ELISA, IF, WB |
|---|---|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Canine, Monkey, Primate |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Anti-FZD6 antibody (orb1672347) was raised against a peptide corresponding to 14 amino acids in the central domain of human FZD6. The immunogen is located within 50-550 amino acids of FZD6. |
| Target | FZD6 |
| Molecular Weight | Predicted: 79kD Observed: 95 kD (Post-translational modification: 2 N-linked glycosylations) |
| Purification | FZD6 Antibody is affinity chromatography purified via peptide column. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | FZD6 Antibody is supplied in PBS containing 0.02% sodium azide. |
| Concentration | 1 mg/mL |
| Disclaimer | For research use only |
Alternative Names
−FZD6 Antibody: Frizzled-6, Fz-6, hFz6
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WB Validation in Human, Mouse and Rat Cell Lines. Loading: 15 µg of lysate. Antibodies: FZD6 orb1672347, 2 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
Immunofluorescence Validation of FZD6 in Daudi Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed Daudi cells labeling FZD6 with orb1672347 at 10 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
Immunofluorescence Validation of FZD6 in Mouse Lung. Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse lung labeling FZD6 with orb1672347 at 10 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
Immunofluorescence Validation of FZD6 in Rat Lung. Immunofluorescent analysis of 4% paraformaldehyde-fixed rat lung labeling FZD6 with orb1672347 at 10 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
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Protocol Information
WB
Western Blot (IB, immunoblot)
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
FZD6 Antibody (orb1672347)
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