FZD3 Rabbit Polyclonal Antibody

• Catalog Number: orb156979
• Category: Antibodies
• Description: FZD3 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Mouse, Rat
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant human FZD3 protein (23-205/666aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-1000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 74 kDa
• Target: FZD3
• UniProt ID: Q9NPG1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Frizzled 3; Frizzled 3 seven transmembrane spannin
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Protein: brain (Rat) lysates at 30 ug, Primary: Anti-FZD3 (orb156979) at 1:300, Secondary: HRP conjugated Goat Anti-Rabbit IgG (orb572747) at 1:3000, ECL excitated the fluorescence, Predicted band size: 74kD, Observed band size: 74kD, 25kD.

Protein: pancreas (mouse) lysates at 30 ug, Primary: Anti-FZD3 (orb156979) at 1:300, Secondary: HRP conjugated Goat-Anti-Rabbit IgG (orb572747) at 1:5000, ECL excitated the fluorescence, Predicted band size: 74kD, Observed band size: 74kD.

Sample: Lane 1: Human SW480 cell Lysates, Lane 2: Human U-2 OS cell Lysates, Lane 3: Human A431 cell Lysates, Lane 4: Human SH-SY5Y cell Lysates, Primary: Anti-FZD3 (orb156979) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 74kDa, Observed band size: 70kDa.

Tissue/Cell: human lung carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-FZD3 Polyclonal Antibody, Unconjugated (orb156979) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Tissue/Cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-FAD3 Polyclonal Antibody, Unconjugated (orb156979) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Tissue/Cell: rat kidney tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-FZD3 Polyclonal Antibody, Unconjugated (orb156979) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.