FZD1 Antibody (Center)

• Catalog Number: orb1931482
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Target: This FZD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 367-396 amino acids from the Central region of human FZD1.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• UniProt ID: Q9UP38
• MW: 71158 Da
• Tested applications: FC, IHC-P, WB
• Dilution range: WB: 1:1000, IHC-P: 1:10~50, FC: 1:10~50
• Antibody Type: Primary Antibody
• Clone Number: RB14325
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Frizzled-1, Fz-1, hFz1, FzE1, FZD1
• Research Area: Cancer Biology, Pharmacology & Drug Discovery, Sig
• Note: For research use only
• NCBI: NP_003496.1
• Expiration Date: 12 months from date of receipt.

Flow cytometric analysis of NCI-H292 cells using FZD1 Antibody (Center) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with FZD1 antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot analysis of anti-FZD1 Antibody (center) in Hela cell line lysates (35 ug/lane).FZD1 (arrow) was detected using the purified Pab.