FXYD6 Antibody (C-term)

SKU: orb1927154

Description

Purified Rabbit Polyclonal Antibody (Pab)

Images & Validation

• Tested Applications: FC, IHC-P, WB
• Dilution Range: WB - 1:1000, IHC-P - 1:25, FC - 1:25
• Reactivity: Human, Mouse, Rat

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: BTD60282
• Immunogen: This FXYD6 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 81-115amino acids from the C-terminal region of human FXYD6.
• Target: FXYD6 (HGNC:4030)
• Molecular Weight: 10542 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

FXYD domain-containing ion transport regulator 6, Phosphohippolin, FXYD6

Immunohistochemical analysis of paraffin-embedded H. liver section using FXYD6 Antibody (C-term). Diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Immunohistochemical analysis of paraffin-embedded M. kidney section using FXYD6 Antibody (C-term). Diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Flow cytometric analysis of SH-SY5Y cells using FXYD6 Antibody (C-term) (green) compared to an isotype control of rabbit IgG (blue). Diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.

All lanes: Anti-FXYD6 Antibody (C-term) at 1:1000 dilution. Lane 1: Human cerebellum tissue lysate. Lane 2: Human brain tissue lysate. Lane 3: SH-SY5Y whole cell lysate. Lane 4: Mouse brain tissue lysate. Lane 5: Mouse cerebellum tissue lysate. Lane 6: Rat brain tissue lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 11 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

Gene Symbol

FXYD6 (HGNC:4030)

UniProt

Q9H0Q3