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| Catalog Number | orb1927154 |
|---|---|
| Category | Antibodies |
| Description | FXYD6 Antibody (C-term) |
| Clonality | Polyclonal |
| Species/Host | Rabbit |
| Isotype | Rabbit IgG |
| Conjugation | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.05% (V/V) Proclin 300. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| UniProt ID | Q9H0Q3 |
| MW | 10542 Da |
| Tested applications | FC, IHC-P, WB |
| Dilution range | WB - 1:1000 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Note | For research use only |

Immunohistochemical analysis of paraffin-embedded H. liver section using FXYD6 Antibody (C-term). Diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Immunohistochemical analysis of paraffin-embedded M. kidney section using FXYD6 Antibody (C-term). Diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Flow cytometric analysis of SH-SY5Y cells using FXYD6 Antibody (C-term) (green) compared to an isotype control of rabbit IgG (blue). Diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.

All lanes: Anti-FXYD6 Antibody (C-term) at 1:1000 dilution. Lane 1: Human cerebellum tissue lysate. Lane 2: Human brain tissue lysate. Lane 3: SH-SY5Y whole cell lysate. Lane 4: Mouse brain tissue lysate. Lane 5: Mouse cerebellum tissue lysate. Lane 6: Rat brain tissue lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 11 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
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