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Item 1 of 4
FUT6 Antibody (Center)
Catalog Number: orb1930757
| Catalog Number | orb1930757 |
|---|---|
| Category | Antibodies |
| Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Clone Number | RB23931 |
| Tested applications | FC, IHC-P, WB |
| Reactivity | Human |
| Isotype | Rabbit IgG |
| Antibody Type | Primary Antibody |
| Dilution range | WB: 1:1000, WB: 1:1000, IHC-P: 1:50~100, FC: 1:10~50 |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Conjugation | Unconjugated |
| MW | 41860 Da |
| Target | This FUT6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 102-130 amino acids from the Central region of human FUT6. |
| UniProt ID | P51993 |
| NCBI | NP_001035791.1, NP_000141.1 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | Alpha-(1,3)-fucosyltransferase 6, Fucosyltransfera Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
FUT6 Antibody (Center) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
FUT6 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded bladder carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the FUT6 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Western blot analysis of FUT6 Antibody (Center) in HL-60 cell line lysates (35 ug/lane). FUT6 (arrow) was detected using the purified Pab.
Anti-FUT6 Antibody (Center) at 1:1000 dilution + Hela whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 42 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
