Fusin rabbit pAb

• Catalog Number: orb765255
• Category: Antibodies
• Description: Rabbit polyclonal antibody to CXCR4
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Immunogen: The antiserum was produced against synthesized peptide derived from human CXCR4. AA range:300-349
• MW: 36
• Tested applications: ELISA, IF, WB
• Dilution range: Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-CXCR4 antibody, anti-C-X-C chemokine receptor
• Note: For research use only

Western blot analysis of lysates from 1) Hela, 2) mouse-brain cells, (Green) primary antibody was diluted at 1:1000, 4°C over night, Dylight 800 secondary antibody was diluted at 1:10000, 37°C 1 hour. (Red) Tubulin β Monoclonal Antibody (5G3) antibody was diluted at 1:5000 as loading control, 4°C over night, Dylight 680 secondary antibody was diluted at 1:10000, 37°C 1 hour.

Western Blot analysis of various cells using Fusin Polyclonal Antibody diluted at 1:1000.

Western Blot analysis of MOUSE-BRAIN cells using Fusin Polyclonal Antibody diluted at 1:1000.

Immunofluorescence analysis of A549 cells, using CXCR4 Antibody. The picture on the right is blocked with the synthesized peptide.