Fusin rabbit pAb Antibody

SKU: orb765255

Description

Rabbit polyclonal antibody to CXCR4

Images & Validation

• Tested Applications: ELISA, IF, WB
• Dilution range: Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.
• Reactivity: Human, Mouse, Rat

Key Properties

• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human CXCR4. AA range:300-349
• Molecular Weight: 36
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

Anti-CXCR4 antibody, anti-C-X-C chemokine receptor type 4 antibody, anti-CXC-R4 antibody, anti-CXCR-4 antibody, anti-FB22 antibody, anti-Fusin antibody, anti-HM89 antibody, anti-LCR1 antibody, anti-Leukocyte-derived seven transmembrane domain receptor antibody, anti-LESTR antibody, anti-NPYRL antibody, anti-Stromal cell-derived factor 1 receptor antibody, anti-SDF-1 rece antibody

Western blot analysis of lysates from 1) Hela, 2) mouse-brain cells, (Green) primary antibody was diluted at 1:1000, 4°C over night, Dylight 800 secondary antibody was diluted at 1:10000, 37°C 1 hour. (Red) Tubulin β Monoclonal Antibody (5G3) antibody was diluted at 1:5000 as loading control, 4°C over night, Dylight 680 secondary antibody was diluted at 1:10000, 37°C 1 hour.

Western Blot analysis of various cells using Fusin Polyclonal Antibody diluted at 1:1000.

Western Blot analysis of MOUSE-BRAIN cells using Fusin Polyclonal Antibody diluted at 1:1000.

Immunofluorescence analysis of A549 cells, using CXCR4 Antibody. The picture on the right is blocked with the synthesized peptide.