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FURIN/PCSK3 Antibody

SKU: orb1248388

Description

FURIN/PCSK3 Antibody

Images & Validation

Tested ApplicationsELISA, WB
ReactivityHuman
Predicted ReactivityMouse, Rat
Application Notes
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:In transfected HEK293 transiently expressing Human Furin (myc and DYKDDDDK tagged), a band of approx. 110kDa is observed. No bands are observed in mock-transfected HEK293 and the same band is observed using anti-myc antibody. Recommended concentration, 0.5-1µg/mL.

Key Properties

Antibody TypePrimary Antibody
HostGoat
ClonalityPolyclonal
ImmunogenThe immunogen for this antibody is: C-RTKRDVYQEPTDP
TargetFURIN / PCSK3
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration500 ug/mL
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

FURIN, furin (paired basic amino acid cleaving enzyme), FUR, PACE, PCSK3, SPC1, FES upstream region, dibasic processing enzyme, furin, furin, membrane associated receptor protein, paired basic amino acid cleaving enzyme (furin, membrane associated receptor protein), proprotein convertase subtilisin/kexin type 3

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FURIN/PCSK3 Antibody

HEK293 lysate (10 ug protein in RIPA buffer) overexpressing Human Furin with C-terminal MYC tag probed with orb1248388 (1 ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with orb1248388 (1 ug/ml) in Lane B.

UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

FURIN/PCSK3 Antibody (orb1248388)

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0.1 mg
$ 920.00
DispatchUsually dispatched within 5-10 working days
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