FUNDC1 Antibody (HRP)

SKU: orb55189

Description

Rabbit polyclonal antibody against FUNDC1 conjugated to HRP.

Research Area

Cancer Biology, Cell Biology, Metabolism Research, Signal Transduction

Images & Validation

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Tested Applications	IHC, WB
Dilution Range	WB (1:1000); IHC (1:50)
Reactivity	Human, Mouse, Rat
Application Notes	A 1:1000 dilution was sufficient for detection of FUNDC1 in 15 µg of rat liver cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.

Key Properties

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Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic peptide from the N-terminal of Human FUNDC1
Target	FUNDC1
Molecular Weight	17 kDa
Purification	Peptide Affinity Purified
Conjugation	HRP

Storage & Handling

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Storage	Conjugated antibodies should be stored according to the product label
Buffer/Preservatives	73.64mM Carbonate, 54.55mM Ethanolamine, 45.45mM Cyanoborohydride, 18.18mM Sodium Hydroxide, 0.23mM Citrate
Concentration	1 mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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FUNDC1, FUN14 domain-containing protein 1

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Immunohistochemistry analysis using Rabbit Anti-FUNDC1 Polyclonal Antibody. Tissue: Brain. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-FUNDC1 Polyclonal Antibody at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 10X. HRP-DAB Detection.

Western blot analysis of Mouse, Rat Liver cell lysates showing detection of ~17.2 kDa FUNDC1 protein using Rabbit Anti-FUNDC1 Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Liver cell lysates. Lane 3: Rat Liver cell lysates. Load: 15 μg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-FUNDC1 Polyclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~17.2 kDa.

Quick Database Links

Gene Symbol

FUNDC1

UniProt

Q8IVP5

NCBI Gene ID

139341

RefSeq (Protein):NP_776155.1

UniProt Details

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No UniProt data available

NCBI Gene Details

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No NCBI Gene data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_776155.1

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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