FUNDC1 Antibody (FITC)

SKU: orb55188

Description

Rabbit polyclonal antibody against FUNDC1 conjugated to FITC.

Research Area

Cancer Biology, Cell Biology, Metabolism Research, Signal Transduction

Images & Validation

• Tested Applications: IHC, WB
• Dilution Range: WB (1:1000); IHC (1:50)
• Reactivity: Human, Mouse, Rat
• Application Notes:
  A 1:1000 dilution was sufficient for detection of FUNDC1 in 15 µg of rat liver cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide from the N-terminal of Human FUNDC1
• Target: FUNDC1
• Molecular Weight: 17 kDa
• Purification: Peptide Affinity Purified
• Conjugation: FITC

Storage & Handling

• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 640.91mM DMSO, 136.36 mM Ethanolamine, 126.89 mM chlorides, 9.09mM phosphates, 9.09mM NaHCO3
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

FUNDC1, FUN14 domain-containing protein 1

Immunohistochemistry analysis using Rabbit Anti-FUNDC1 Polyclonal Antibody. Tissue: Brain. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-FUNDC1 Polyclonal Antibody at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 10X. HRP-DAB Detection.

Western blot analysis of Mouse, Rat Liver cell lysates showing detection of ~17.2 kDa FUNDC1 protein using Rabbit Anti-FUNDC1 Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Liver cell lysates. Lane 3: Rat Liver cell lysates. Load: 15 μg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-FUNDC1 Polyclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~17.2 kDa.

Quick Database Links

Gene Symbol

FUNDC1

UniProt

Q8IVP5

NCBI Gene ID

139341

RefSeq (Protein):NP_776155.1