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Catalog Number | orb55186 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody against FUNDC1 conjugated to APC. |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ICC, IF, IHC |
Reactivity | Human, Mouse, Rat |
Immunogen | Synthetic peptide from the N-terminal of Human FUNDC1 |
Concentration | 1 mg/ml |
Dilution range | WB (1:1000); IHC (1:50) |
Conjugation | APC |
MW | 17 kDa |
Target | FUNDC1 |
Entrez | 139341 |
UniProt ID | Q8IVP5 |
NCBI | NP_776155.1 |
Storage | Conjugated antibodies should be stored according to the product label |
Buffer/Preservatives | 95.64mM Phosphate, 2.48mM MES and 2mM EDTA |
Alternative names | FUNDC1 antibody, FUN14 domain-containing protein 1 Read more... |
Note | For research use only |
Application notes | A 1:1000 dilution of SPC-638 was sufficient for detection of FUNDC1 in 15 µg of rat liver cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody. |
Expiration Date | 12 months from date of receipt. |
Immunohistochemistry analysis using Rabbit Anti-FUNDC1 Polyclonal Antibody. Tissue: Brain. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-FUNDC1 Polyclonal Antibody at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 10X. HRP-DAB Detection.
Western blot analysis of Mouse, Rat Liver cell lysates showing detection of ~17.2 kDa FUNDC1 protein using Rabbit Anti-FUNDC1 Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Liver cell lysates. Lane 3: Rat Liver cell lysates. Load: 15 μg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-FUNDC1 Polyclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~17.2 kDa.