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Catalog Number | orb1788492 |
---|---|
Category | Antibodies |
Description | Functional Myosin IIA (non-muscle) (heavy chain) Antibody, mAb (recombinant) |
Clonality | Monoclonal |
Clone Number | SF9 |
Tested applications | ELISA, ICC, WB |
Reactivity | Drosophila, Human, Mouse, Rat |
Antibody Type | Primary Antibody |
Concentration | 1mg/ml |
Conjugation | Unconjugated |
UniProt ID | Q9UKX2 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | Cellular Myosin Heavy Chain, Type A; Myosin Heavy Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Human Myosin IIA (non-muscle) (heavy chain) is detected by immunocytochemistry using anti-myosin IIA (non-muscle) (rec.) (SF9). Method:HeLa cells are grown in standard culture conditions, fixed with methanol, and incubated with anti-Myosin IIA (non-muscle) (rec.) (SF9) (1mg /ml in PBS-BSA). After incubation for 30 min at RT and several washes in PBS, cells are treated with a goat anti-human (Cy3) antibody for 30 min at RT, washed and mounted in Moewiol. Nuclei are stained with DAPI.