FTO rabbit pAb Antibody

SKU: orb766638

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Description

Rabbit polyclonal antibody to FTO

Images & Validation

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Tested Applications	ELISA, WB
Dilution Range	Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Reactivity	Human, Mouse, Rat

Key Properties

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Clonality	Polyclonal
Isotype	IgG
Immunogen	The antiserum was produced against synthesized peptide derived from human FTO. AA range:19-68
Molecular Weight	58
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
Concentration	1 mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Anti-FTO antibody, anti-KIAA1752 antibody, anti-Alpha-ketoglutarate-dependent dioxygenase FTO antibody, anti-Fat mass and obesity-associated protein antibody

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Western Blot analysis of extracts from K562 cells, using FTO Polyclonal Antibody. Secondary antibody was diluted at 1:20000.

Western blot analysis of lysates from brain tissue, using FTO antibody.

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Protocol Information

Western Blot (IB, immunoblot)

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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