FTCD Antibody

• Catalog Number: orb1247975
• Category: Antibodies
• Description: FTCD Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, FC, IF, IHC, WB
• Predicted Reactivity: Bovine, Rat
• Reactivity: Human, Mouse, Porcine
• Immunogen: The immunogen for this antibody is: CLREQGRGKDQPGRL
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 60kDa band observed in Human Liver lysates and approx. 55-60kDa in Mouse and Pig Liver lysates, and also in preliminary testing of Rat Liver, Human Kidney and U2OS lysates (calculated MW of 58.9kDa according to Human NP_006648.1, Mouse NP_543121.1 and Pig NP_999440.1). Recommended concentration: 0.03-0.3ug/ml. Primary incubation 1 hour at room temperature.Immunohistochemistry:In paraffin embedded Human Liver shows distinct staining of membranes and cytoplasm in a selection of hepatocytes. Recommended concentration: 3-5ug/ml.Immunofluorescence: Strong expression of the protein seen in the cytoplasm, membranes and plasma membranes of HeLa and in the plasma membranes of HepG2 cells. Recommended concentration: 10ug/ml. Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration: 10ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: FTCD
• UniProt ID: O95954
• NCBI: NP_996848.1, NP_006648.1, NP_001307341.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: FTCD, LCHC1, formiminotransferase cyclodeaminase,
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 60kDa band observed in Human Liver lysates and approx. 55-60kDa in Mouse and Pig Liver lysates, and also in preliminary testing of Rat Liver, Human Kidney and U2OS lysates (calculated MW of 58.9kDa according to Human NP_006648.1, Mouse NP_543121.1 and Pig NP_999440.1). Recommended concentration: 0.03-0.3ug/ml. Primary incubation 1 hour at room temperature.Immunohistochemistry:In paraffin embedded Human Liver shows distinct staining of membranes and cytoplasm in a selection of hepatocytes. Recommended concentration: 3-5ug/ml.Immunofluorescence: Strong expression of the protein seen in the cytoplasm, membranes and plasma membranes of HeLa and in the plasma membranes of HepG2 cells. Recommended concentration: 10ug/ml. Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration: 10ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1247975 (0.1 ug/ml) staining of Human (A), (0.03 ug/ml) of Mouse (B) and (0.3 ug/ml) of Pig (C) Liver lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1247975 (3.75 ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1247975 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).

orb1247975 Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing plasma membrane staining.

orb1247975 Flow cytometric analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line).