FSTL1 Antibody (C-term)

• Catalog Number: orb1938905
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB28536
• Tested applications: FC, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:1000, WB: 1:1000, WB: 1:1000, IHC-P: 1:50~100, FC: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 34986 Da
• Target: This FSTL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 280-308 amino acids from the C-terminal region of human FSTL1.
• UniProt ID: Q12841
• NCBI: NP_009016.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Follistatin-related protein 1, Follistatin-like pr
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

All lanes: Anti-FSTL1 Antibody (C-term) at 1:1000 dilution. Lane 1: A549 whole cell lysate. Lane 2: human heart lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 35 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-FSTL1 Antibody (C-term) at 1:1000 dilution. Lane 1: Hela whole cell lysate. Lane 2: human placenta lysate. Lane 3: human heart lysate. Lane 4: mouse heart lysate. Lane 5: rat heart lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 35 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

FSTL1 Antibody (C-term) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

FSTL1 antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the FSTL1 antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

FSTL1 Antibody (C-term) western blot analysis in A549 cell line lysates (35 ug/lane). This demonstrates the FSTL1 antibody detected the FSTL1 protein (arrow).