FREM1 Antibody

• Catalog Number: orb1538574
• Category: Antibodies
• Description: FREM1 Antibody
• Target: FREM1
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS containing 0.02% Sodium Azide. PBS containing 0.02% Sodium Azide
• Purification: Peptide Column Affinity Chromatography
• Immunogen: A 15 AA synthetic peptide near the C-Terminus of human FREM1. The immunogen is located within the last 50 AA of FREM1.
• Tested applications: ELISA, ICC, IF, IHC, IHC-P, WB
• Dilution range: ELISA, ICC, IF, IHC, IHC-P (10 µg/ml), WB (0.5 - 1 µg/ml)
• Application notes: Further information: FREM1 antibody can be used for detection of FREM1 by Western blot at 0.5 - 1 ug/mL. Antibody can also be used for immunocytochemistry starting at 20 ug/mL. For immunofluorescence start at 20 ug/mL. Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Western Blot:
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: FREM1, BNAR, C9orf143, C9orf145, C9orf154, TRIGNO2
• Note: For research use only

Anti-FREM1 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

Immunocytochemistry of FREM1 in K562 cells with FREM1 antibody at 20 ug/ml.

Immunofluorescence of FREM1 in K562 cells with FREM1 antibody at 20 ug/ml.

Western blot analysis of FREM1 in K562 cell lysate with FREM1 antibody at (A) 0.5 and (B) 1 ug/ml.