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Catalog Number | orb1935874 |
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Category | Antibodies |
Description | Purified Rabbit Polyclonal Antibody (Pab) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Clone Number | RB14254 |
Tested applications | IHC-P, WB |
Reactivity | Human |
Isotype | Rabbit IgG |
Antibody Type | Primary Antibody |
Dilution range | WB: 1:1000, IHC-P: 1:10~50 |
Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
Conjugation | Unconjugated |
MW | 29093 Da |
Target | This FRAT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 244-274 amino acids from the C-terminal region of human FRAT1. |
UniProt ID | Q92837 |
NCBI | NP_005470.2 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | Proto-oncogene FRAT1, Frequently rearranged in adv Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of lysates from SK-BR-3, K562 cell line (from left to right), using FRAT1 Antibody (C-term). Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with FRAT1 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.