Foxp3 Antibody

SKU: orb1247485

Description

Foxp3 Antibody

Research Area

Cancer Biology

Images & Validation

• Tested Applications: ELISA, FC, IHC
• Reactivity: Mouse
• Predicted Reactivity: Rat
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:4000.Immunohistochemistry:In paraffin embedded Mouse Thymus shows nuclear staining in some patches of thymocytes. Recommended concentration: 2-4µg/mL.Flow Cytometry: Flow cytometric analysis of NIH3T3 cells. Recommended concentration: 10µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: KRSQRPNKCSNP
• Target: Foxp3
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Foxp3, forkhead box P3, sf, JM2, scurfin, scurfy, RP23-54C14.1, OTTMUSP00000017952, OTTMUSP00000017961, OTTMUSP00000017962

In paraffin embedded Mouse Spleen shows patches of stained cells in the Germinal Centre. Recommended concentration, 2-4 ug/ml.

orb1247485 Flow cytometric analysis of paraformaldehyde fixed NIH3T3 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line).

Quick Database Links

Gene Symbol

Foxp3

UniProt

Q99JB6

RefSeq (Protein):NP_473380.1