Foxp3 Antibody

• Catalog Number: orb1247485
• Category: Antibodies
• Description: Foxp3 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, FC, IHC
• Predicted Reactivity: Rat
• Reactivity: Mouse
• Immunogen: The immunogen for this antibody is: KRSQRPNKCSNP
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:4000.Immunohistochemistry:In paraffin embedded Mouse Thymus shows nuclear staining in some patches of thymocytes. Recommended concentration: 2-4ug/ml.Flow Cytometry: Flow cytometric analysis of NIH3T3 cells. Recommended concentration: 10ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: Foxp3
• UniProt ID: Q99JB6
• NCBI: NP_473380.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: Foxp3, forkhead box P3, sf, JM2, scurfin, scurfy,
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:4000.Immunohistochemistry:In paraffin embedded Mouse Thymus shows nuclear staining in some patches of thymocytes. Recommended concentration: 2-4ug/ml.Flow Cytometry: Flow cytometric analysis of NIH3T3 cells. Recommended concentration: 10ug/ml.
• Expiration Date: 12 months from date of receipt.

In paraffin embedded Mouse Spleen shows patches of stained cells in the Germinal Centre. Recommended concentration, 2-4 ug/ml.

orb1247485 Flow cytometric analysis of paraformaldehyde fixed NIH3T3 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line).