FOXP3 Antibody (C-term)

• Catalog Number: orb1931451
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB17111
• Tested applications: FC, IHC-P, WB
• Reactivity: Human, Mouse
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:1000, IHC-P: 1:10~50, FC: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 47244 Da
• Target: This FOXP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 283-311 amino acids from the C-terminal region of human FOXP3.
• UniProt ID: Q9BZS1
• NCBI: NP_001107849.1, NP_054728.2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Forkhead box protein P3, Scurfin, FOXP3, IPEX
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

FOXP3 Antibody (C-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Formalin-fixed and paraffin-embedded human lymph tissue reacted with FOXP3 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot analysis of FOXP3 Antibody (C-term) in 293 (lane 1), Jurkat cell line (lane 2) and mouse liver tissue (lane 3) lysates (35 ug/lane). FOXP3 (arrow) was detected using the purified Pab.