FOXP2 Antibody

• Catalog Number: orb1247629
• Category: Antibodies
• Description: FOXP2 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Bovine, Canine, Feline, Mouse, Porcine, Zebrafish
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-DEVEYQKRRSQKIT
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 85kDa band observed in Human Brain (Cerebellum and Frontal Cortex) lysates and in Rat Brain lysates (calculated MW of 82.6kDa according to NP_683696.1). Recommended concentration: 0.3-1ug/ml. An additional band of unknown identity was also consistently observed at 28-30kDa. This band was successfully blocked by incubation with the immunising peptide. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Prostate and Placenta. Recommended concentration: 2.5ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: FOXP2
• UniProt ID: O15409
• NCBI: NP_001166237.1, NP_683698.2, NP_683697.2, NP_683696.2, NP_055306.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: FOXP2, forkhead box P2 , CAGH44, DKFZp686H1726, SP
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 85kDa band observed in Human Brain (Cerebellum and Frontal Cortex) lysates and in Rat Brain lysates (calculated MW of 82.6kDa according to NP_683696.1). Recommended concentration: 0.3-1ug/ml. An additional band of unknown identity was also consistently observed at 28-30kDa. This band was successfully blocked by incubation with the immunising peptide. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Prostate and Placenta. Recommended concentration: 2.5ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1247629 (0.3 ug/ml) staining of Human Brain (Cerebellum) lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1247629 (2.5 ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1247629 (2.5 ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.