FOXP1 Recombinant Rabbit Monoclonal Antibody

SKU: orb559207

Description

FOXP1 Recombinant Rabbit Monoclonal Antibody

Research Area

Cancer Research, Cell Biology, Forkhead box (FOX) proteins, Tumor Suppressors

Images & Validation

• Tested Applications: FC, ICC, IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:50-200, IHC-F=1:50-200, IF=1:50-200, Flow-Cyt=1:50-100, ICC/IF=1:50-200
• Reactivity: Human, Mouse
• Predicted Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Isotype: IgG
• Clone No.: 15E2
• Immunogen: A synthesized peptide derived from human FOXP1 (350-401/677aa)
• Target: FOXP1
• Molecular Weight: 79 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

FLJ23741; Fork head related protein like B; Forkhead box P1; Forkhead box protein P1; 12CC4; foxp1; FOXP1_MOUSE; FOX P1; FOXP 1; Glutamine rich factor 1; hFKH1B; HSPC215; MGC12942; MGC88572; MGC99551; QRF 1; QRF1.

Blocking buffer: 5% NFDM/TBST, Primary ab, dilution: 1:2000, Primary ab incubation condition: 2 hours at room temperature, Secondary ab: Goat Anti-Rabbit IgG H&L (HRP), Lysate: 1: HeLa, 2: MCF-7, 3: Daudi, Protein loading quantity: 20 µg, Exposure time: 60 s, Predicted MW: 75kDa, Observed MW: 50-95 kDa.

Tissue: human colon carcinoma, Section type: Formalin fixed & Paraffin embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0, Primary ab, dilution: 1:1000, Primary ab incubation condition: 1 hour at room temperature, Secondary ab: Anti-Rabbit and Mouse Polymer HRP, Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb559207.

Tissue: human colon, Section type: Formalin fixed & Paraffin embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0, Primary ab, dilution: 1:1000, Primary ab incubation condition: 1 hour at room temperature, Secondary ab: Anti-Rabbit and Mouse Polymer HRP, Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb559207.

Tissue: mouse colon, Section type: Formalin fixed & Paraffin embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0, Primary ab, dilution: 1:1000, Primary ab incubation condition: 1 hour at room temperature, Secondary ab: Anti-Rabbit and Mouse Polymer HRP, Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb559207.

Tissue: rat colon, Section type: Formalin fixed & Paraffin embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0, Primary ab, dilution: 1:1000, Primary ab incubation condition: 1 hour at room temperature, Secondary ab: Anti-Rabbit and Mouse Polymer HRP, Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb559207.

Quick Database Links

Gene Symbol

FOXP1

UniProt

Q9H334