FOXP1 Recombinant Rabbit Monoclonal Antibody

• Catalog Number: orb559207
• Category: Antibodies
• Description: FOXP1 Recombinant Rabbit Monoclonal Antibody
• Target: FOXP1
• Clonality: Recombinant
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Immunogen: Recombinant human FOXP1 protein, around C-terminal 150aa
• UniProt ID: Q9H334
• MW: 79 kDa
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:1000-2000, IHC-P=1:100-500, IHC-F=1:50-200), IF=1:50-200)
• Antibody Type: Recombinant Antibody
• Clone Number: 15E2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: FLJ23741; Fork head related protein like B; Forkhe
• Note: For research use only

Blocking buffer: 5% NFDM/TBST, Primary ab, dilution: 1:2000, Primary ab incubation condition: 2 hours at room temperature, Secondary ab: Goat Anti-Rabbit IgG H&L (HRP), Lysate: 1: HeLa, 2: MCF-7, 3: Daudi, Protein loading quantity: 20 µg, Exposure time: 60 s, Predicted MW: 75kDa, Observed MW: 50-95 kDa.

Tissue: human colon carcinoma, Section type: Formalin fixed & Paraffin embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0, Primary ab, dilution: 1:1000, Primary ab incubation condition: 1 hour at room temperature, Secondary ab: Anti-Rabbit and Mouse Polymer HRP, Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb559207.

Tissue: human colon, Section type: Formalin fixed & Paraffin embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0, Primary ab, dilution: 1:1000, Primary ab incubation condition: 1 hour at room temperature, Secondary ab: Anti-Rabbit and Mouse Polymer HRP, Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb559207.

Tissue: mouse colon, Section type: Formalin fixed & Paraffin embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0, Primary ab, dilution: 1:1000, Primary ab incubation condition: 1 hour at room temperature, Secondary ab: Anti-Rabbit and Mouse Polymer HRP, Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb559207.

Tissue: rat colon, Section type: Formalin fixed & Paraffin embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0, Primary ab, dilution: 1:1000, Primary ab incubation condition: 1 hour at room temperature, Secondary ab: Anti-Rabbit and Mouse Polymer HRP, Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb559207.