FOXO6 Antibody (Center)

SKU: orb1937552

Description

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Images & Validation

• Tested Applications: IF, WB
• Dilution Range: IF - 1:10-50, WB - 1:1000
• Reactivity: Mouse

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: BTD54480
• Immunogen: This FOXO6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 154-181 amino acids from the Central region of human FOXO6. Antigen Region: 154-181 aa.
• Target: FOXO6
• Molecular Weight: 50595 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Forkhead box protein O6, FOXO6

Fluorescent confocal image of U251 cell stained with FOXO6 Antibody (Center). U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with FOXO6 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7 units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). FOXO6 immunoreactivity is localized to Cytoplasm and Nucleus significantly.

FOXO6 Antibody (Center) western blot analysis in mouse heart tissue lysates (35 ug/lane). This demonstrates the FOXO6 antibody detected the FOXO6 protein (arrow).

Quick Database Links

Gene Symbol

FOXO6

UniProt

A8MYZ6