FOXO3 Antibody (N-term)

SKU: orb1936776

Description

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Cell Biology

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: WB - 1:1000, IHC-P - 1:10-50
• Reactivity: Human
• Predicted Reactivity: Mouse

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: BTD55413
• Immunogen: This FOXO3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human FOXO3. Antigen Region: 1-30 aa.
• Target: FOXO3 (HGNC:3821)
• Molecular Weight: 71277 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Forkhead box protein O3, AF6q21 protein, Forkhead in rhabdomyosarcoma-like 1, FOXO3, FKHRL1, FOXO3A

All lanes: Anti-FOXO3 Antibody (N-term) at 1:1000 dilution. Lane 1: MCF-7 whole cell lysate. Lane 2: SH-SY5Y whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 71 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

FOXO3 Antibody (N-term) western blot analysis in Hela, MCF-7 cell line lysates (35 ug/lane). This demonstrates the FOXO3 antibody detected the FOXO3 protein (arrow).

FOXO3 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of FOXO3 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Quick Database Links

Gene Symbol

FOXO3 (HGNC:3821)

UniProt

O43524

RefSeq (Protein):NP_963853.1, NP_001446.1